Trial toward the Regeneration of Periodontal Tissues around Titanium Implant

Trial toward the Regeneration of Periodontal Tissues around Titanium Implant
26 June 2003
K. MATSUMURA, S. TSUTSUMI, N. IKUMI, N. NAKAJIMA, C. PENG, and S.-H. HYON, Kyoto University, Japan
IADR
Objectives: In order to require the functional recovery of the dental implant, a trial of regenerating PDL around implant was carried out by culturing PDL cells on a poly(ethylene-co-vinyl alcohol)(EVA) coated implant. In this report, the guided tissue regeneration membrane was used to prevent the downgrowth of the gingival tissue around the implant. And the cementum is the highly calcified tissue and is believend to be the important tissue that is related to metabolism of the collagen fiber. So the osteoblastic differentiation of PDL cells on hydroxyapatite formed EVA is investigated. Methods:Our hybrid implant was prepared as follows. The titanium implant was oxidized by 30% hydrogen peroxide at 60 ¨?C for 24 h. EVA was coated onto the implant and ozone treatment was carried out for introduction of carboxyl groups. The collagen sponge was immobilized around the implant by freeze-drying method. PDL cells were seeded onto the implant and implanted in dog's alveolar bone and covered with the biodegradable membrane. After 3 months of implantation, the implants and their surrounding tissue were recovered and the histological specimens were prepared. Results: The collagen fiber around implant was seemed to be vertically to the alveolar bone similar to the one around natural tooth. Hydroxyapatite was formed on EVA films by alternately soaking in the CaCl2 and the Na2HPO4 solutions. The calcium concentration of the culture medium at culture on the apatite formed EVA was lower than that on the tissue culture dish. This result may show that the PDL cells differentiated to osteoblastic cells by the stimulation of hydroxyapatite and may generate the calcified tissue. Conclusion: The results suggested that the novel hybrid implant has a potentiality to regenerate periodontal tissues containing of PDL and cementum.