Toxicity evaluation of root canal sealers in vitro - Root Canal (Endodontics) Articles

Toxicity evaluation of root canal sealers in vitro
Published article online:
11 Oct 2007
Received 2 April 2007; accepted 2 July 2007
To cite this article: G. Lodienė, E. Morisbak, E. Bruzell, D. ?rstavik
Toxicity evaluation of root canal sealers in vitro
International Endodontic Journal (OnlineEarly Articles).
doi:10.1111/j.1365-2591.2007.01321.x
Blackwell Synergy

G. Lodienė1,21NIOM-Nordic Institute of Dental Materials, Haslum, Norway2Department of Dental and Oral Pathology, Faculty of Odontology, Kaunas University of Medicine, Kaunas, Lithuania, E. Morisbak11NIOM-Nordic Institute of Dental Materials, Haslum, Norway, E. Bruzell11NIOM-Nordic Institute of Dental Materials, Haslum, Norway & D. ?rstavik33Department of Endodontics, Institute of Clinical Dentistry, Faculty of Dentistry, University of Oslo, Institute of Clinical Dentistry, Oslo, Norway

Greta Lodienė, Department of Dental and Oral Pathology, Faculty of Odontology, Kaunas University of Medicine, Eiveniu 2, LT-50009 Kaunas, Lithuania (Tel.: +370 37 326681; fax: +370 37 295237; e-mail: Greta Lodienė, Department of Dental and Oral Pathology, Faculty of Odontology, Kaunas University of Medicine, Eiveniu 2, LT-50009 Kaunas, Lithuania (Tel.: +370 37 326681; fax: +370 37 295237; e-mail: lodieneg@gmail.com).
Lodienė G, Morisbak E, Bruzell E, ?rstavik D. Toxicity evaluation of root canal sealers in vitro. International Endodontic Journal.).
Lodienė G, Morisbak E, Bruzell E, ?rstavik D. Toxicity evaluation of root canal sealers in vitro. International Endodontic Journal.

Abstract

Aim To compare the toxicity of methacrylate resin-based root canal sealers with sealers based on epoxy resin and silicone by two-well established cell culture methods.

Methodology Specimens of AH Plus, EndoREZ, RoekoSeal and Epiphany were prepared for direct contact in the Millipore filter diffusion test and as extracts in the MTT assay. Mouse fibroblasts (L929) were used as toxicity targets. Differences in cytotoxicity between fresh and set specimens and between the extracts of root canal sealers were determined by t-test (P < 0.05).

Results In the filter diffusion test, freshly mixed Epiphany and AH Plus were rated severely toxic and RoekoSeal and EndoREZ nontoxic. When set, Epiphany was moderately toxic, whereas AH Plus, RoekoSeal and EndoREZ were nontoxic. Epiphany was significantly more toxic than RoekoSeal and EndoREZ (P < 0.05). In the MTT assay with set specimens, Epiphany was rated severely toxic; AH Plus and RoekoSeal slightly toxic; and EndoREZ nontoxic. Epiphany was significantly more toxic than the other three materials in this test (P < 0.001).

Conclusion The multi-methacrylate resin-based (Epiphany) root canal sealer was significantly more toxic to L-929 cells than the silicone-based Roeko Seal and the single methacrylate-based EndoREZ root canal sealers. AH Plus showed intermediate toxicity.

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