The effect of the surface modification of titanium using a recombinant fragment of fibronectin and vitronectin on cell behavior

The effect of the surface modification of titanium using a recombinant fragment of fibronectin and vitronectin on cell behavior
Received 15 October 2004; accepted 25 January 2005. Available online 8 March 2005.
Young Kua, Chong-Pyoung Chunga and Jun-Hyeog Jang
Biomaterials
Abstract
The surface of titanium implants is in direct contact with host tissue and plays a critical role in determining biocompatibility. Fibronectin (FN) and vitronectin (VN) are major cell adhesive proteins found in the extracellular matrix (ECM) of various tissues, and in circulating blood. The aim of this study was to evaluate the engineered biomimetic surface of titanium by using recombinant fragment of FN8ÇƒÏ10 and VNNTD that contains the binding site for integrins. MC3T3-E1 cells seeded upon the FN8-10-coated titanium showed a marked increase in cell adhesion, proliferation, and differentiation over VNNTD-coated titanium. In addition, we confirmed that the surface properties of titanium prefer for FN8ÇƒÏ10 over VNNTD (p<0.05) in protein adhesion. These results suggest that the FN8ÇƒÏ10-modified titanium surface can be used to improve the osseointegration of titanium implants by enhancing bone formation.