Effects of RGD peptide grafting to titanium dental implants on the adhesion of human gingival fibroblasts and epithelial cells

Effects of RGD peptide grafting to titanium dental implants on the adhesion of human gingival fibroblasts and epithelial cells
Received 18 October 2004; revised 12 November 2004. Available online 21 February 2005.
BaoHong Zhaoa, c, d, WeiMing Tianb, HaiLan Fengc, In-Seop Leed, , and FuZhai Cuib
Current Applied Physics
ScienceDirect
aDepartment of Prosthodontics, School of Stomatology, China Medical University, Shenyang 110002, China
bBiomaterials Laboratory, Department of Materials Science & Engineering, Tsinghua University, Beijing 100084, China
cDepartment of Prosthodontics, School of Stomatology, Peking University, Beijing 100081, China
dInstitute of Physics & Applied Physics, Yonsei Center for Nano Technology, Yonsei University, Seoul 120-749, Republic of Korea
Abstract
The aim of this study is to test the adhesion of human gingival fibroblasts (HGFs) and epithelial cells (HGEs) to titanium surface by RGD grafting for dental implant, and to obtain an effective perimucosal seal of soft tissue to the implant. RGD peptides were covalently attached to the titanium surface by CDI activation method to improve the adhesion of gingival cells to the titanium dental implants. HGFs and HGEs were inoculated on commercially pure (CP) titanium and RGD-grafted titanium. The number of attached and proliferated cells was quantified by morphometric analysis using immunofluorescence. The results showed that the bioactive peptide RGD was grafted on CP titanium by CDI activation method. The RGD grafting exhibited that the number of attached and proliferated fibroblasts and epithelial cells were higher than that on CP titanium. The RGD-grafting is advantageous to gingival cells adherence to the surface of titanium implant.
Keywords: Dental implant; RGD; Fibroblasts; Epithelial cells; Cell adhesion
PACS: 81.05.-Bx; 81.20.-n; 82.30.-b
Corresponding author. Tel.: + 82 2 2123 4806; fax: + 82 2 313 3537
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