Behavior of SaOS-2 Cells Cultured on Different Titanium Surfaces

Behavior of SaOS-2 Cells Cultured on Different Titanium Surfaces
September 2003
By L. Postiglione, G. Di Domenico, L. Ramaglia, S. Montagnani, S. Salzano, F. Di Meglio, L. Sbordone, M. Vitale, and G. Rossi
Journal of Dental Research
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Surface properties may affect the clinical outcome of titanium dental implants. The aim of the present study was to investigate the effects of 3 different titanium surfacesÇƒÓsmooth (S), sandblasted (SB), and titanium plasma-sprayed (TPS)ÇƒÓon proliferation, differentiation, and apoptosis of human osteoblast-like cells, SaOS-2. Cell proliferation was significantly (p < 0.05) higher on the S surface, and synthesis of extracellular matrix proteins was more abundant on TPS and SB than on S surfaces. Analysis of integrin receptors showed a higher expression of 2, 5, V?¸3, and ?¸1 on TPS as compared with SB and S surfaces. An increase in alkaline phosphatase activity was detected only on SB and TPS surfaces. Analysis of cell apoptosis did not demonstrate any significant difference among the 3 different surfaces. The results indicate that titanium surface topography affects proliferation and differentiation of osteoblast-like SaOS-2 cells, suggesting that surface properties might be important for bone response around dental implants in vivo.