ANALYSIS OF OSTEOBLAST-LIKE MG63 CELLS' RESPONSE TO A ROUGH IMPLANT SURFACE BY MEANS OF DNA MICROARRAY

ANALYSIS OF OSTEOBLAST-LIKE MG63 CELLS' RESPONSE TO A ROUGH IMPLANT SURFACE BY MEANS OF DNA MICROARRAY
October 2003
by Francesco Carinci, MD; Furio Pezzetti, PhD; Stefano Volinia, PhD; Francesca Francioso, PhD; Diego Arcelli, PhD;
Jlenia Marchesini, PhD; Luca Scapoli, PhD; Adriano Piattelli, MD
Journal of Oral Implantology
Please visit the web site to view the article in its entirety.
Abstract
Several features of the implant surface, such as composition, topography, roughness, and energy, play a relevant role in implant integration with bone. Little is known about the structural and chemical surface properties that may influence biological responses. Expression profiling by DNA microarray is a molecular technology that allows the analysis of gene expression in a cell system. By using DNA microarrays containing 19 200 genes, we identified several genes whose expression was significantly down-regulated in osteoblast-like cell line MG63 on a new implant surface (titanium pull spray superficial [TPSS] surface, Oralplant, Cordenons, PN, Italy). The differentially expressed genes cover a broad range of functional activities: (1) signaling transduction, (2) translation, (3) cell cycle regulation, (4) structural and metabolic functions, and (5) apoptosis. It was also possible to detect some genes whose functions are unknown. The data reported can be relevant to better understand the role of the type of surface on the molecular mechanism of implant osseointegration and as a model for comparing other materials.